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Analysis of the genetic diversity of 20 Calocedrus macrolepis samples by RAPD DNA chloroplast markers

Last modified on 20/11/2010 at 1:43:00 AM. Total 3798 views.

In this study, RAPD and cpDNA markers were used to assess genetic diversity of 20 Calocedrus macrolepis samples collected from three different locations (Ha Tay, Quang Binh and Lam Dong). Among fifteen RAPD primers, there were 9 primers that revealed polymorphism with PIC value (Polymorphic Information Content) varying from 0.08 (RA40) to 0.31 (RA46), no primer had PIC value ≥ 0.5. A total of 56 DNA fragments were generated but only 22 DNA fragments showed polymorphism (approximately 39.2%). Genetic similarity coefficients between samples ranged from 0.686 (QB2 and HT2; QB1 and LD6) to 1.0 (QB5 and QB6) and was used for constructing a phenetic dendrogram using UPGMA analysis. The pattern of grouping in the dendrogram divided 20 Calocedrus macrolepis samples into 3 main groups. The first group includes 7 samples that were collected in Ha Tay, the second group is formed from 7 samples from Lam Dong and the third group involves 6 samples of Quang Binh province. Six primer pairs of cpDNA were used to assess genetic diversity of 20 Calocedrus macrolepis samples there were four primer pairs that generated DNA fragments from 0.5 kb to 1.0 kb. However, non-polymorphic bands were generated, even digested by 9 different restriction enzymes.

Vu Thi Thu Hien, Tran Thi Viet Thanh, Dinh Thi Phong
Vietnam Natural Museum, VAST 
Le Anh Tuan, Phi Hong Hai
Forest Tree Improvement Research Centre, FSIV
(Proceedings of 3rd National Scientific Conference on Ecology and Biological Resources, Hanoi 22th October 2009 - IEBR)

anhtai.bvn

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