This investigation was conducted on the series of flowers of L. racemosa collected from Phu Tan Commune, Phu Vang District, Thua Thien Hue Province, Vietnam to determine pollen fertility of this species. Pollen fertility tests in vitro by aceto-carmine staining technique were used in evaluating pollen fertility (Reijieli R. Rigamoto et al, 2002). Using dissecting forceps and needle, the anthers were collected from the flowers. Mature anthers were crushed and subsequently transferred in micro-eppendorf containing 60µl 5% aceto-carmine. The eppendorf solution was centrifuged to allow pollen to fully dissociate from the anthers and a drop of sample was gently placed onto slide. The slide was then observed under a light microscope. For each slide ten randomly selected fields were observed under the 10x objective (100x magnification). The size of pollen grains was measured under a light microscope fitted with an ocular micrometer (John K. Kelly et al, 2002). Pollen production status of the flowers was determined. The mean diameter of viable pollen grains is 24.98 0.43µm and the mean diameter of non-viable pollen grains is 18.96 ± 0.51µm. The mean pollen fertility is 71.70 ± 0.79%. The number of pollen per flower is 4912.13 ± 124.97.
Key words: Lumnitzera racemosa Wild. 1803, pollen fertility, pollen production, viable pollen, non-viable pollen.
Tran Quoc Dunge
Institute of Resource - Environment and Bio-Technology, Hue University
Nguyen Khoa Lan
Hue Pedagogy University
(Proceedings of 3rd National Scientific Conference on Ecology and Biological Resources, Hanoi 22th October 2009 - IEBR)