Summary:
Somatic embryogenic callus derived from nucellar cells have been induced by ovule culture of local Citrus cultivars including orange Van du (C.sinensis), mandarins Quyt Chum,Duong Canh( C. reticulata) and Cam Sanh ( Citrus nobilis).Ovaries and immature fruits (1-8 weeks after anthesis) are surface disinfected and cut open under aseptical condition.The ovules are excised and cultured on the MT culture media with different concentrations of Kinetine and BAP.The embryogenic callus (EC) induction rates are quite different and depending both on genotypes and cultured media.The induced callus have been propagated by periodically cultures on solid and liquid media.The suitable media for EC induction and propagation have been developed for each studied cultivars.Embryogenic callus were profusely developed into well-formulated somatic embryos which are developed into microplants in MT medium without growth regulator.The percentage of plants survive after transfered to the soil is very high.
Embryogenic callus is currently used in our laboratory for different researches: embryogenesis in bioreactor,tetraploid induction in vitro by colchicine treatement,development of disease-free materials and for study on obtaining seedless somaclones derived from local cultivars.
Ha Thi Thuy, Tran Thi Hanh,Nguyen Hong Chien, Do Nang Vinh
Institute of Agricultural Genetics
Vu Van Vu
Hanoi National University
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